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KMID : 0378019790220110069
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New Medical Journal
1979 Volume.22 No. 11 p.69 ~ p.73
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Changes of Hematocrit, Reduced and Oxidized Glutathione of the Rat Blood Following the Repeated Administration of Ethanol
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Abstract
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In an attempt to observe the changes of hematocrit (Hct), reduced glutathione (GHS)} and oxidized glutathione (GSSG) levels of the blood following the repeated administration of ethanol, a daily dose of 1. Oml of 33% alcohol per 100gm BW was orally ingested to albino rats repeatedly for 40 days, and the experiment was performed at 5 days interval. from 20th day of the alcohol ingestion.
The rat was sacrificed by a heavy blow to the head, and thorax was immediately opend.. The blood sample for both Hct, blood GSH and GSSG was drawn from the thoracic cavity with the heart puncture. The Hct was determined by the conventional Hct centrifuge and
reader. The blood GSH was measured by the Ellman´s method, and the blood GSSG by thee electrolytic reduction method of Dohan and Woodward. The control was set in which the same amout of water in place of alcohol was ingested, and otherwise the procedure wass identical with the experimental group.
The results were compared with the normal value, and summarized as follows. Where the Hct was 45.1±1.77% (mean±SE) in the normal group, significantly higher values were obtained when alcohol was repeatedly administered with the return to the normal level on 40 days. The level of blood GSH showed significantly high values in the alcohol intubated group, while the blood GSSG level was decreased significantly in the alcohol group comparing with the normal. Both blood GSH and GSSG values returned to the normal level on 40 days of alcohol intubation.
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